Detection of ROS and NO in Vivo and Vitro
|
||
|
Detection of CP•-radicals and NO-Hb complexes in blood. (A) EPR spectra of CP•-radical
(spectrum a) and NO-Hb complex (spectrum b) recorded at liquid nitrogen
temperature. (B) Separation of NO-Hb and CP•-radical signals using power
saturation. Pure NO-Hb signal can be obtained at high power (32 mW or more) when
CP• signal is saturated (spectrum d). Pure CP• spectrum is obtained by
subtraction of spectrum d from mixed spectrum obtained at 1 mW(spectrum c). (C)
Power saturation curves of CP• and NO-Hb signals.
Kozlov A.V. et all. FRBM, 2003: Vol. 34, pp. 1555–1562 |
||
|
||
|
Generation of NO and RONS in blood of control
animals (n=9) and animals receiving LPS (n= 8). (A) Generation of
RONS was followed by CP•-radical signal. The rate of RONS generation in control
and LPS challenged animals was 27±3 nM/min and 68±4 nM/min, respectively. (B)
Generation of NO was followed by detection of NO-Hb signal. The rate of NO
generation in control animals and in animals receiving LPS was 1±3 nM/min and
89±8 nM/min, respectively.
Kozlov A.V. et all. FRBM, 2003: Vol. 34, pp. 1555–1562 |
Main Menu
Products
Featured [more]
Starter-Kit, Accessories 772.00 EUR 617.00 EUR
20% off
Specials [more]
Starter-Kit, Accessories
772.00 EUR 617.00 EUR
20% off
