CPH
Effective, partially cell permeable and non-toxic spin probe for the detection
in vitro and in vivo of superoxide radical and other reactive oxygen species
such ONOO-. The spin adduct is resistant to reduction by vitamin C and thiols.
Half-life time of spin adduct in biological environment amount to 4h. Samples in
frozen condition (-80 °C or liquid nitrogen) can be stored up to 6 months.

Rerferences

1. Fink B, Dikalov S et al. A new approach for extracellular spin trapping of nitroglycerin-induced formation of superoxide radicals both in vitro and in vivo. Free Radical Biology and Medicine 2000; 28(1):121-128. (PDF)
2. Dikalov S, Fink B et al. Comparison of glyceryl trinitrate-induced with pentaerythrityl tetranitrate-induced in vivo formation of suproxide radicals: Effect of vitamin C. Free Radical Biology and Medicine 1999; 27(1/2):170-176. (PDF)
3. Wyche KE, Wang SS, Griendling KK, Dikalov SI, Austin H, Rao S, Fink B, Harrison DG, Zafari AM. C242T CYBA polymorphism of the NADPH oxidase is associated with reduced respiratory burst in human neutrophils. Hypertension 2004; 43(6):1246-1251. (PDF)